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La matrice d'acétate de cellulose sert à la fois de support solide pour le test immunoenzymatique, de revêtement protecteur pour l'électrode de mesure et de barrière laissant diffuser sélectivement la substance active (le peroxyde d'hydrogène produit par la glucose-oxydase) vers cette électrode. Une première partie des travaux a porté sur l'étude de la diffusion du peroxyde d'hydrogène à travers ces membranes et notamment sur l'influence de l'épaisseur et des conditions de préparations. L'effet de l'hydrolyse par la soude sur la perméabilité à différents électrolytes de tailles variées a également été testé. Afin de permettre le greffage de protéines sur cette matrice une méthode originale d'activation a été mise au point. Elle consiste en une oxydation par le metaperiodate de sodium des résidus alpha-diol précédemment régénérés lors d'une première étape d'hydrolyse des esters acétyles par la soude. Deux méthodes de couplage des anticorps ont été testées, la première faisant intervenir le SMCC pour le greffage de fragment Fab', la seconde utilisant le succinic dihydrazine."}],"tag":300},{"ind2":0,"ind1":" ","subfield":[{"code":"b","content":"Thèse de Doctorat"},{"code":"c","content":"Génie Enzymatique, Bioconversion, Microbiologie"},{"code":"e","content":"Compiègne"},{"code":"d","content":1992}],"tag":328},{"ind2":" ","ind1":" ","subfield":[{"code":7,"content":"ba0yba0"},{"code":"t","content":"Achievement and characterization of a cellulose acetate support for an immunoenzymatic test with amperometric detection"}],"tag":441},{"ind2":1,"ind1":" ","subfield":[{"code":3,"content":188957804},{"code":7,"content":"ba0yba04"},{"code":"a","content":"Balloy"},{"code":"b","content":"Vincent"},{"code":"f","content":"19..-...."},{"code":4,"content":"070"}],"tag":501},{"ind2":1,"ind1":" ","subfield":[{"code":3,"content":"075723050"},{"code":7,"content":"ba0yba04"},{"code":"a","content":"Boitieux"},{"code":"b","content":"Jean-Louis"},{"code":"f","content":"19..-...."},{"code":4,"content":727}],"tag":501},{"ind2":2,"ind1":0,"subfield":[{"code":3,"content":"026570564"},{"code":7,"content":"ba0yba04"},{"code":"a","content":"Université de Technologie de Compiègne"},{"code":"c","content":"1972-..."},{"code":4,"content":295}],"tag":511},{"ind2":2,"ind1":0,"subfield":[{"code":3,"content":"16971652X"},{"code":7,"content":"ba0yba04"},{"code":"a","content":"École doctorale Sciences pour l'ingénieur"},{"code":"c","content":"Compiègne"},{"code":4,"content":996}],"tag":511},{"ind2":" ","ind1":" ","subfield":[{"code":3,"content":"092467067"},{"code":"a","content":"Acétate de cellulose"},{"code":2,"content":"rameau"}],"tag":606},{"ind2":" ","ind1":" ","subfield":[{"code":3,"content":"027812316"},{"code":"a","content":"Perméabilité"},{"code":2,"content":"rameau"}],"tag":606},{"ind2":" ","ind1":" ","subfield":[{"code":3,"content":"027360067"},{"code":"a","content":"Oxydation"},{"code":2,"content":"rameau"}],"tag":606},{"ind2":" ","ind1":" ","subfield":[{"code":3,"content":"098259938"},{"code":"a","content":"Acide succinique"},{"code":2,"content":"rameau"}],"tag":606},{"ind2":" ","ind1":" ","subfield":[{"code":3,"content":"029682495"},{"code":"a","content":"Biocapteurs"},{"code":2,"content":"rameau"}],"tag":606},{"ind2":" ","ind1":" ","subfield":[{"code":3,"content":153613386},{"code":"a","content":"Immunocapteurs"},{"code":2,"content":"rameau"}],"tag":606},{"ind2":" ","ind1":" ","subfield":[{"code":7,"content":"ba0yba0"},{"code":3,"content":"027253139"},{"code":"a","content":"Thèses et écrits académiques"},{"code":2,"content":"rameau"}],"tag":608},{"ind2":" ","ind1":0,"subfield":{"code":"a","content":"Oxydation des sucres"},"tag":610},{"ind2":" ","ind1":0,"subfield":{"code":"a","content":"Succinique dihydrazyne"},"tag":610},{"ind2":" ","ind1":0,"subfield":{"code":"a","content":"Glucose oxydase"},"tag":610},{"ind2":" ","ind1":0,"subfield":{"code":"a","content":"SDH"},"tag":610},{"ind2":" ","ind1":0,"subfield":{"code":"a","content":"SMCC"},"tag":610},{"ind2":" ","ind1":" ","subfield":[{"code":"a","content":610},{"code":2,"content":"TEF"}],"tag":686},{"ind2":3,"ind1":" ","subfield":[{"code":"a","content":"FR"},{"code":"b","content":"Abes"},{"code":"c","content":20231218},{"code":"g","content":"AFNOR"}],"tag":801}],"controlfield":[{"tag":"001","content":247839310},{"tag":"003","content":"http://www.idref.fr/247839310"},{"tag":"004","content":20200708},{"tag":"005","content":20231218030933},{"tag":"006","content":"0499"},{"tag":"007","content":"0499"},{"tag":"008","content":"Tr2"}]}}