Note publique d'information : Chromatin is of central importance to gene regulation in eukaryotes. Reflecting this
singular role for chromatin, numerous approaches have evolved in the laboratory over
the past three decades to study chromatin structure and its alterations. Methods of
investigating chromatin remodeling, whether in changes in nucleosome structure or
position with respect to the incorporated DNA or in histone modifications, have progressed
rapidly over the recent years. In Chromatin Remodeling: Methods and Protocols, expert
researchers contribute chapters which include methods for investigating chromatin
remodeling in vitro and in vivo, in yeast, plants, and mammalian cells, and at local
and global levels. Both gene-specific and genome-wide approaches are covered, and
in recognition of the increasing prevalence of the latter type of study, the final
two chapters focus on bioinformatic/computational approaches to analyzing genome-wide
data on chromatin structure. Written in the highly successful Methods in Molecular
Biology™ series format, the chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive
and essential, Chromatin Remodeling: Methods and Protocols serves as a key source
of clear techniques as well as a collection of chapters that can inspire future techniques
in this vital field of study
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